Blueberry anthocyanins extract inhibits acrylamide-induced diverse toxicity in mice by preventing oxidative stress and cytochrome P450 2E1 activation

The intervention effects of blueberry anthocyanins extract (BAE) on the acrylamide (AA)-induced general toxicity, genotoxicity and reproductive toxicity were investigated in a mice model. Compared to the AA group, BAE restored the AA-induced alteration in haematology and serum chemistry, markedly decreased the DNA damage in lymphocyte and liver cells, as well as micronucleus formation in bone marrow cell. Meanwhile, BAE improved sperm motility levels and decreased abnormal sperm rate. Furthermore, BAE inhibited the AA-induced ROS accumulation and glutathione (GSH) depletion, ameliorated the activities of glutathione peroxidase (GPx) and glutathione-S-transferase (GST), improved the protein expression of GPx, GST and gamma-glutamyl cysteine synthase (γ-GCS), and inhibited the cytochrome P4502E1 (CYP2E1) protein expression. Thus, the intervention mechanism of BAE on the AA-induced toxicity possibly attributed to the prevention of AA-induced oxidative stress and CYP2E1 activation. BAE would have potential application for the chemoprevention of the AA toxicity as a nutraceutical or a dietary supplement.