کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7625249 | 1494557 | 2014 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Pilot-scale membrane fractionation of ACE inhibitory and antioxidative peptides from ultrasound pretreated milk protein concentrate hydrolysates
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Pilot-scale membrane fractionation of ACE inhibitory and antioxidative peptides from ultrasound pretreated milk protein concentrate hydrolysates Pilot-scale membrane fractionation of ACE inhibitory and antioxidative peptides from ultrasound pretreated milk protein concentrate hydrolysates](/preview/png/7625249.png)
چکیده انگلیسی
A batch mode, pilot-scale process, involving ultrasound pretreatment (USP), enzymolysis, membrane filtration and drying was investigated for the production of angiotensin converting enzyme (ACE) inhibitory and antioxidative peptides from milk protein concentrate (MPC). The MPC solutions (2% or 5% protein) were hydrolyzed for 2 h with Neutrase at enzyme-to-substrate ratio of 1:50 (w/w) in 50 L batches after USP for 4 min at 800 W. Ultrafiltration (8 and 3.5 kDa membranes) and nanofiltration (0.2 kDa membrane) were done to produce 4 fractions (F1, >8 kDa; F2, 3.5-8 kDa; F3, 0.2-3.5 kDa; and F4, <0.2 kDa). Analyses showed that large amounts of ACE inhibitory peptides were recovered by the 3.5 kDa membrane and concentrated by nanofiltration making F3 ideal for ACE inhibition (IC50 = 0.096 ± 0.010 mg mLâ1) while antioxidative peptides were abundant in F2 with bioactivity not affected by drying method. Initial substrate concentration had no significant effect (P < 0.05) on bioactivity. Furthermore, permeation fluxes were high when a low protein concentration was used. Ultrafiltration achieved peptide rejections rates in the range of 24.0-33.7% and reached 89.6% during nanofiltration. The results indicate that membrane filtration can separate ACE inhibitory and antioxidative peptides in complex protein hydrolysates.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Functional Foods - Volume 7, March 2014, Pages 350-361
Journal: Journal of Functional Foods - Volume 7, March 2014, Pages 350-361
نویسندگان
Hankie Uluko, Shuwen Zhang, Lu Liu, Hongjuan Li, Wenming Cui, Haixiao Xue, Lili Zhao, Yanjun Sun, Jing Lu, Jiaping Lv,