کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7625708 | 1494558 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Nobiletin, a polymethoxy flavonoid, exerts anti-allergic effect by suppressing activation of phosphoinositide 3-kinase
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کلمات کلیدی
HSADNPPBS-TPLCFcεRIHRPBMMCDMEMPI3KPBSFBSBSA - BSADMSO - DMSOPBS containing 0.05% Tween 20 - PBS حاوی 0.05٪ Tween 20PCA - PCA[Ca2+]i - [Ca2 +] ihuman serum albumin - آلبومین سرم انسانیbovine serum albumin - آلبومین سرم گاوPassive cutaneous anaphylaxis - آنافیلاکسی پوستی منفعلstandard deviation - انحراف معیارdimethyl sulphoxide - دی متیل سولفوکسیدdinitrophenol - دینیتروفنلfetal bovine serum - سرم جنین گاوbone marrow-derived mast cell - سلول های مشتق شده از مغز استخوانRBL-2H3 cells - سلولهای RBL-2H3intracellular Ca2+ concentration - غلظت Ca2 + داخل سلولیPhosphatidylinositol 3-kinase - فسفاتیدیلینواستیل 3-کینازphospholipase C - فسفولیپاز Cphosphoinositide 3-kinase - فسفینوزیتید 3-کینازAnti-allergic activity - فعالیت ضد حساسیتDegranulation - قرمزی چشمPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریDulbecco’s modified eagle’s medium - محیط عقاب اصلاح شده DulbeccoNobiletin - نوبیلتینwortmannin - ورتمنینHorseradish peroxidase - پراکسیداز هوررادیشhigh-affinity IgE receptor - گیرنده IgE باکتری
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Nobiletin, a polymethoxy flavonoid, exerts anti-allergic effect by suppressing activation of phosphoinositide 3-kinase Nobiletin, a polymethoxy flavonoid, exerts anti-allergic effect by suppressing activation of phosphoinositide 3-kinase](/preview/png/7625708.png)
چکیده انگلیسی
Nobiletin is a polymethoxy flavonoid present in fruit tissues of citrus species and has been reported to suppress degranulation of RBL-2H3 cells. However, the detailed mechanism is still unknown. In this study the mechanism of suppressive effect of nobiletin on degranulation of RBL-2H3 cells was examined. Nobiletin suppressed degranulation of both RBL-2H3 cells and bone marrow-derived mast cells induced by antigen stimulation. Nobiletin also suppressed degranulation stimulated by the calcium ionophore A23187 and thapsigargin, a specific inhibitor of the Ca2+-dependent ATPase. Elevation of intracellular free Ca2+ concentration was also suppressed by nobiletin when RBL-2H3 cells were stimulated by either antigen or A23187. Immunoblot analysis revealed that the inhibitory effect results from down-regulated phosphorylation of both phosphatidylinositol 3-kinase (PI3K) and phospholipase Cγ1 in the signaling pathways related to degranulation stimulated by antigen. Furthermore, oral administration of nobiletin suppressed vascular permeability in passive cutaneous anaphylaxis model mice. The tendency in all experiments obtained by treating with nobiletin was highly similar to that obtained by wortmannin, a specific inhibitor of PI3K. These findings suggest that nobiletin suppresses Ca2+ influx and consequently exerts the anti-allergy activity in a similar way to wortmannin.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Functional Foods - Volume 6, January 2014, Pages 606-614
Journal: Journal of Functional Foods - Volume 6, January 2014, Pages 606-614
نویسندگان
Sayo Onishi, Kosuke Nishi, Sho Yasunaga, Ayako Muranaka, Kazutaka Maeyama, Ayumu Kadota, Takuya Sugahara,