کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7625954 | 1494578 | 2018 | 18 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination and pharmacokinetic study of Enasidenib in rat plasma by UPLC-MS/MS
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Enasidenib, an oral product for treating Acute Myeloid Leukemia, has been approved by FDA in Aug, 2017. In this study, we set up an ultra-performance liquid chromatography-mass spectrometry (UPLC-MS/MS) method for measuring Enasidenib and imatinib (internal standard, IS), simultaneously. Enasidenib and imatinib were separated on an ACQUITY UPLC BEH C18 Column (2.1â¯mmâ¯Ãâ¯50â¯mm, 1.7â¯Î¼m, 132â¯Ã
). Mass detection was carried out by electrospray ionization in the position mode, and the multiple reaction monitoring transitions were m/zâ¯474.23â¯ââ¯456.17 and m/zâ¯494.30â¯ââ¯394.20 for Enasidenib and imatinib, respectively. Linearity (2â¯ââ¯500â¯ng·mLâ1, R2â¯>â¯0.999), precision and accuracy (REâ¯<â¯Â±â¯15%), extraction recovery (â¥â¯96.69%), matrix effect (â¥â¯96.47%) and stability (REâ¯<â¯Â±â¯10%) were validated which demonstrated the robustness of our method. This rapid, efficient and reliable UPLC-MS/MS method shows specificity and repeatability of Enasidenib in rat plasma and can be used in further pharmacokinetic studies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 157, 5 August 2018, Pages 165-170
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 157, 5 August 2018, Pages 165-170
نویسندگان
Ni-hong Pang, Qian Liu, Xiang-ran Lu, Su-fen Yang, Dong-dong Lin, Guo-xin Hu,