کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7625985 | 1494577 | 2018 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Validated LC-MS/MS method for the determination of amlodipine enantiomers in rat plasma and its application to a stereoselective pharmacokinetic study
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A rapid and sensitive method was established to determine amlodipine enantiomers using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Stereoselective separation was performed on CHIRALCEL OZ-RH column (150â¯mmâ¯Ãâ¯4.6â¯mm i.d., 5â¯Î¼m) with acetonitrile-water (10â¯mM ammonium acetate, 0.5% ammonia solution) (95:5, v/v) at a flow rate of 0.5â¯mL/min. The substances were detected by mass spectrometer equipped with an electrospray ionization source interface in positive ion mode. Multiple reaction monitoring was selected with the transition of the m/z 409.1â¯ââ¯238.0 for amlodipine enantiomers and m/z 237.0â¯ââ¯194.1 for carbamazepine (IS) respectively. Calibration curves were linear at the range of 0.9375-120â¯ng/mL for both isomers with râ¯>â¯0.99, while using a lower sample volume (50â¯Î¼L) compared with previously reported enantiospecific methods The accuracy was at the range of 84.1-119.0% for R-amlodipine, and 87.4-118.2% for S-amlodipine, respectively. The within- and between-run precision (CV%) was within 10% in all cases for both enantiomers. Enantiomers were stable under different conditions, e.g. processed sample, short-term, residue, long-term and freeze/thaw. The LC-MS/MS method was successfully applied in pharmacokinetic study of amlodipine enantiomers in rats. It was observed the concentration of the S- amlodipine was significantly higher than that of the R-amlodipine in racemate-treated group. And there was no significant difference in the pharmacokinetic profiles of the S-amlodipine between the 10â¯mg/kg racemate- and 5â¯mg/kg S-amlodipine-treated groups. In addition, it was the first time to find that the main pharmacokinetic parameters (AUC(0-t), AUC(0-â) and Cmax) of R-amlodipine were significantly lower in the 5â¯mg/kg R-amlodipine-treated group compared with the racemate-treated group.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 158, 5 September 2018, Pages 74-81
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 158, 5 September 2018, Pages 74-81
نویسندگان
Lijuan Wang, Wenxia Liu, Zunjian Zhang, Yuan Tian,