Rutaecarpine and evodiamine selected as β1-AR inhibitor candidates using β1-AR/CMC-offline-UPLC/MS prevent cardiac ischemia-reperfusion injury via energy modulation

- A β1-AR/CMC-offline-UPLC/MS method was developed.
- Potential β1-AR antagonists were firstly selected from E. rutaecarpa (Juss) Benth.
- Rutaecarpine and evodiamine could regulate glycolipid substrate metabolism of ischemia/reperfusion heart.