An untargeted metabolomics-driven approach based on LC-TOF/MS and LC-MS/MS for the screening of xenobiotics and metabolites of Zhi-Zi-Da-Huang decoction in rat plasma

- An untargeted metabolomics approach was developed for metabolism research of TCMP.
- XCMS platform combined with OPLS-DA can clearly display the interest tR-m/z pairs.
- A total of 61 ZZDHD-related xenobiotics and metabolites were characterized.
- The metabolite profiles of multiple components of ZZDHD in rats were explored.