Quantitation of pregabalin in dried blood spots and dried plasma spots by validated LC-MS/MS methods

▶ Sensitive LC-MS/MS methods for pregabalin determination in DBS and DPS. ▶ Derivatization of pregabalin with n-propyl chloroformate/n-propanol combination. ▶ DBS concentration correction was suggested, due to hematocrit effect. ▶ The validated methods were applied to samples obtained from epileptic patients. ▶ Correlation of DBS and DPS levels for estimation of pregabalin distribution into RBC.