Development and validation of a liquid chromatography-tandem mass spectroscopy method for simultaneous determination of (+)-(13aS)-deoxytylophorinine and its pharmacologically active 3-O-desmethyl metabolite in rat plasma

- A method was developed for simultaneous determination of CAT and its metabolite S-4.
- We develop an assay with short run time and low LLOQ.
- The method showed excellent chromatographic resolution.
- The validated method was successfully applied to a pharmacokinetic study in rats.