Multiplexed LC-MS/MS method for the simultaneous quantitation of three novel hepatitis C antivirals, daclatasvir, asunaprevir, and beclabuvir in human plasma

- Simultaneous quantitation of three novel HCV drugs in human plasma by LC-MS/MS.
- The validation results demonstrate the method is accurate, precise, and reproducible.
- Cross-validation results demonstrated method equivalency with those for single drug.
- The plasma method was used for pharmacokinetic assessment in many clinical studies.
- Multiplexed LC-MS/MS method is time- and cost-effective, and maintains high performance in sample analysis.