Development and validation of liquid chromatography tandem mass spectrometry method for simultaneous quantification of first line tuberculosis drugs and metabolites in human plasma and its application in clinical study

- We develop and validate a LC-MS/MS method to quantify TB drugs and metabolites.
- The method passes FDA guidance on bioanalytical method development.
- We apply the method in a pharmacokinetic study of healthy volunteers.
- The methodology enables a better understanding of the TB drug metabolism profiling.
- The methodology provides a way of phenotyping the acetylation of isoniazid.