Development, validation and clinical application of a LC-MS/MS method for the simultaneous quantification of hydroxychloroquine and its active metabolites in human whole blood

- Hydroxychloroquine and its metabolites were quantified in whole blood by LC-MS/MS.
- The method was fully validated for all compounds according to EMA guidelines.
- The assay was suitable for concentrations.
- 20,000 ng/mL after sample dilution.
- The method was successfully applied to a blood sample after massive poisoning.
- Monitoring metabolites in treatment or overdose needs to be assessed.