Derivatization for the simultaneous LC/MS quantification of multiple neurotransmitters in extracellular fluid from rat brain microdialysis

- Novel derivatization for the rapid LC/MS quantification of neurotransmitters.
- Enable simultaneous quantization of amino acids in brain microdialysis samples.
- LOQ was single digit nM with linear dynamic a range up to 1000 nM and coefficient >0.990.
- This method applied to brain microdialysis study in tetrodotoxin (TTX) treated rat.
- TTX inhibition of synaptic GABA, glutamate and glycine release was quantified.