Absolute protein quantification of clinically relevant cytochrome P450 enzymes and UDP-glucuronosyltransferases by mass spectrometry-based targeted proteomics

- Simultaneous LC-MS/MS-based quantification of 13 relevant metabolizing enzymes.
- Comprehensive method validation for all aforementioned enzymes.
- Application to quantify enzymes in human liver and intestinal microsomes.
- First protein data on intestinal metabolizing enzymes by targeted proteomics.