The analysis of carbohydrates in milk powder by a new “heart-cutting” two-dimensional liquid chromatography method

- A novel method was proposed to determine glucose, galactose, fructose, saccharose and lactose in milk powder.
- The method is based on “heart cutting” two-dimensional liquid chromatography (LC-LC).
- The effective parameters in the two dimensional HPLC system were optimized.
- A conventional one dimensional chromatography method was proposed to compare with the new two dimensional chromatography method.