Validated LC-MS/MS assay for quantitative determination of deoxypodophyllotoxin in rat plasma and its application in pharmacokinetic study

- A LC-MS/MS method was first developed and validated for the determination of deoxypodophyllotoxin in rat plasma.
- Good peak shape and sensitivity with acceptable matrix effect could be obtained.
- It is the first pharmacokinetic study of the intravenous administration of DPT in rats.
- The pharmacokinetic parameters were obtained through two-compartment intravenous models analysis.