Identification of 20(S)-protopanaxatriol metabolites in rats by ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry and nuclear magnetic resonance spectroscopy

In vivo metabolism of 20(S)-protopanaxatriol (PPT) was first investigated by UPLC-Q/TOF-MS and NMR. A total of 22 metabolites including oxidative products, glucuronidation products and cysteine-conjugates was detected and identified.