Investigation of monoclonal antibody fragmentation artifacts in non-reducing SDS-PAGE

- First study that systematically compared IAM/NEM in SDS-PAGE artifact inhibition.
- The artifact inhibition activity of both agents is concentration dependent.
- 5 mM NEM achieves the same inhibition effect as 40 mM IAM under same conditions.
- NEM retained activity after prolonged sample heating, whereas IAM did not.
- NEM has a better inhibition effect than IAM on all tested IgG4 proteins.