A stability-indicating, ion-pairing, reversed-phase liquid chromatography method for studies of daunorubicin degradation in i.v. infusion fluids

HPLC analysis of a solution of daunorubicin (0.4 mg/mL) with different chromatographic conditions: (A) Column: Synergi MAX-RP C12 4 μm (150 mm × 4.6 mm I.D.). Isocratic elution: 10 mM acid acetic buffer (pH 4.8) and acetonitrile (62:38, v/v). (B) Column: Polar-RP column 4 μm (150 mm × 4.6 mm I.D.). Isocratic elution: 6.2 mM NFPA in water and acetonitrile (65:35, v/v). (C) Column: Synergi MAX-RP C12 4 μm (150 mm × 4.6 mm I.D.). Isocratic elution: 6.2 mM NFPA in water and acetonitrile (65:35, v/v). Flow rate: 1 mL min−1; injection volume: 20 μL; UV detection at 254 nm.