High resolution reversed phase analysis of recombinant monoclonal antibodies by ultra-high pressure liquid chromatography column coupling

- High-resolution RPLC is a valuable strategy for monoclonal antibody analysis.
- Long columns packed with sub-2 μm particles provide very high separation power.
- Adsorption of intact mAb at the surface of the stationary phase remained minimal.
- Peak capacities of >700 were achieved for digested mAb using 450 mm long column.