Comparative metabolism study of β-lapachone in mouse, rat, dog, monkey, and human liver microsomes using liquid chromatography-tandem mass spectrometry

- Comparative metabolism of β-lapachone was investigated in liver microsomes.
- The chemical structures of β-lapachone metabolites were characterized using LC-MS/MS.
- A total of 6 metabolites were detected.
- An O-methylated metabolite (M5) was tentatively identified.