Hydrophilic interaction chromatography with aerosol-based detectors (ELSD, CAD, NQAD) for polar compounds lacking a UV chromophore in an intravenous formulation

► HILIC is used to separate and retain a polar drug and mannitol in an IV matrix. ► Three polar stationary phases were evaluated in the HILIC mode. ► Four aerosol-based detectors were compared and evaluated for validation parameters.