A validated high-performance liquid chromatography method with diode array detection for simultaneous determination of nine flavonoids in Senecio cannabifolius Less.

► A simple, accurate and effective HPLC-DAD method for simultaneous determination of nine flavonoids in S. cannabifolius has been developed for the first time. ► The analytical work is the first report regarding flavonol-5-O-β-d-glucosides in Senecio species. ► The proposed method will likely serve as a useful tool in the quality control of S. cannabifolius.