Development of an enzyme-linked immunosorbent assay for detection of cellular and in vivo LRRK2 S935 phosphorylation

► We established two LRRK2 ELISA's. ► Serine 935 phosphorylation is used as readout for LRRK2 activity. ► One assay can be used to screen for cellular active LRRK2 inhibitors. ► We present an ELISA to measure in vivo pS935 LRRK2 levels. ► With this assay we demonstrate in vivo efficacy of a LRRK2 inhibitor.