Comparative pharmacokinetics study of three anthraquinones in rat plasma after oral administration of Radix et Rhei Rhizoma extract and Dahuang Fuzi Tang by high performance liquid chromatography-mass spectrometry

► The validated LC-MS method was applied to determine three anthraquinone. ► We compare the pharmacokinetics parameters between DH alone and DFT. ► We can better understand the rationality and compatibility of DFT prescription.