Discrimination between glycosylation patterns of therapeutic antibodies using a microfluidic platform, MALDI-MS and multivariate statistics

► Glycosylation patterns were analysed using a microfluidic platform and MALDI-TOF-MS. ► Preparation of 54 parallel samples could be finished in less than 4 h. ► A data set was created to mimic the variation in therapeutic antibody production. ► Glycosylation patterns of four different antibodies were well classified. ► SIMCA of two similar glycosylation patterns gave a well-defined group classification.