Simultaneous determination of imperatorin and its 2 metabolites in dog plasma by using liquid chromatography-tandem mass spectrometry

► 2 metabolites of imperatorin were identified for the first time in dog plasma. ► Imperatorin and its 2 metabolites were simultaneously determined. ► The LC-MS/MS method was sensitive, specific, and accurate. ► Pharmacokinetics of imperatorin and its 2 metabolites showed sex differences.