کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7633816 1494709 2018 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantitative proteomic analysis of deciduous molars during cap to bell transition in miniature pig
ترجمه فارسی عنوان
تجزیه پروتئومیک کمی مولرهای شفاف در طول کلاهبرداری به زنگ زدن در خوک مینیاتوری
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
The third deciduous molar (Dm3) in the mandible of the miniature pigs corresponding to cap stage at E40 and bell stage at E50 (E: embryonic days). Tooth germs of E40 and E50 were pooled separately into two groups. Total peptides were labeled using tandem mass tag (TMT) after protein extraction and digestion with trypsin for proteomic analysis; E40 and E50 labeled with TMT 128 and TMT 129, respectively. The mixture was run on a capillary/nano-HPLC system and separated into sequential fractions. The identification of peptides from the acquired MS/MS spectra were performed using the NCBI protein database search approach. The enriched kyoto encyclopedia of genes and genomes (KEGG) pathways, the protein-protein interaction (PPI) network and the validation of candidate proteins of differentially regulated proteins (DRPs) were constructed.181
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Proteomics - Volume 172, 10 February 2018, Pages 57-67
نویسندگان
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