Probing molecular events associated with early development of thylakoid membranes by comparative proteomics and low temperature fluorescence

In this study, we monitored the light-induced transformation of prolamellar bodies into thylakoid membranes, which is correlated to the biogenesis of photosynthetic apparatus involving a complex cascade of biochemical and structural events. Three stages of thylakoid development classified according to the thylakoid development status (Adam et al., 2011) were studied for biogenesis of photosynthetic apparatus: Stage 1, prothylakoids emerge from the disrupted PLBs; Stage 2, prothylakoids converted into primary thylakoids which were dispersed in the stroma; Stage 3, the continuous grana and stroma thylakoids are formed. The development stage-dependent changes in the proteomic profile of the thylakoids were analyzed by two-dimensional electrophoresis (2-DE). This information was complemented with the steady-state 77 K chlorophyll fluorescence of thylakoids at the corresponding development stage. Together, these analyses allowed us to further understand the molecular processes connected to the formation of functional thylakoid membranes.