Isotopically-coded short-range hetero-bifunctional photo-reactive crosslinkers for studying protein structure

Short-range non-specific photo-reactive hetero-bifunctional crosslinkers ABAS, SDA, and CBS can provide shorter distance constraints with a broader reactivity than homo-bifunctional crosslinkers utilizing NHS-ester alone. Isotopic coding of these crosslinkers additionally allows for the specific detection of the crosslinks when analyzed by MS.41