Highly sensitive detection of hepatitis C virus DNA by using a one-donor-four-acceptors FRET probe

The D-4A FRET probe was formed by incubating the mixture of DNA A1, A2.and D1. Then the fluorescence of donor (FAM) was quenched because of FRET from donor to acceptors (BHQ1) when the probe was formed, and it was recovered due to disintegration of probe structure when the HCV DNA was added.170