The synthesis of surface-glycosylated porous monolithic column via aqueous two-phase graft copolymerization and its application in capillary-liquid chromatography

A facile, flexible process was developed for the preparation of surface-glycosylated porous monolith via aqueous two-phase graft copolymerization of polyethylene glycol diacrylate (PEGDA) and water-soluble dextran (dextran sulfate). The formation of poly(PEGDA) porous skeletons and surface glycosylation were achieved via a one-step process without pre-modification of the polysacharide. Under the optimized conditions, the monolith exhibited a large dry state surface area (greater than 400 m2/g) and homogeneous continuous porous structure. The chromatographic characterization results demonstrate the application of optimized monolithic column in hydrophilic interaction chromatography for polar molecular.267