کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7680846 | 1495816 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Design and validation of a novel immunological test for enterolactone
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کلمات کلیدی
hapten(ELISA)(BSA)(PBS)bovine serum albumin - آلبومین سرم گاوPolyclonal antibodies - آنتیبادیهای پلی کلنالenterolactone - انتولوکتونELISA - تست الیزاHigh resolution mass spectroscopy - طیف سنجی جرمی با وضوح بالاBioavailability - فراهم زیستیphosphate buffer saline - فسفات بافر شورphytoestrogens - فیتواستروژن هاPlasma - پلاسماoptical density - چگالی نوری
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Design and validation of a novel immunological test for enterolactone Design and validation of a novel immunological test for enterolactone](/preview/png/7680846.png)
چکیده انگلیسی
Enterolactone (ENL) is produced by the gut microflora from lignans found in edible plants. ENL is estrogenic with no effect on the E-screen test and is a natural Selected Estrogen Receptor Modulator (SERM) with health interests that have to be checked in clinical studies with bioavailability assessment. Two haptens of ENL were synthesized, with a spacer arm at the C5 position having either 2 or 4 carbon atoms (ENLÎ2 and ENLÎ4, respectively). Hapten coupling to bovine serum albumin (BSA) was characterized by MALDI mass spectrometry. Polyclonal antibodies were obtained against the BSA conjugates. Additional conjugates were generated by coupling to swine thyroglobulin (Thyr). Homologous and heterologous competitive ELISAs were developed with Thyr or BSA conjugates as coating. The best assays were validated on biological samples from mice. Both antibodies exhibited the same IC50 at 1.5 ng mLâ1 with a detection limit below 0.5 ng mLâ1. Most cross-reactions with structurally related lignans were lower than 0.03%. This new assay type is faster, more specific and more reliable than existing ones.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 119, 15 February 2014, Pages 116-124
Journal: Talanta - Volume 119, 15 February 2014, Pages 116-124
نویسندگان
Svitlana Shinkaruk, Edith Pinot, Valérie Lamothe, Jean-Marie Schmitter, Laurie Baguenard, Bernard Bennetau, Catherine Bennetau - Pelissero,