کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7681956 1495822 2013 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Simultaneous determination of glucose, d-gluconic, 2-keto-d-gluconic and 5-keto-d-gluconic acids by ion chromatography-pulsed amperometric detection with column-switching technique
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Simultaneous determination of glucose, d-gluconic, 2-keto-d-gluconic and 5-keto-d-gluconic acids by ion chromatography-pulsed amperometric detection with column-switching technique
چکیده انگلیسی
A simple ion chromatographic (IC) method for simultaneous determination of glucose, d-gluconic acid (DGA), 2-keto-d-gluconic acid (2-KDG) and 5-keto d-gluconic acid (5-KDG) was proposed, with pulsed amperometric detection (PAD) and column-switching technique. Using this technique, the four compounds were detected simultaneously in a short time with strongly retained compounds (2-KDG and 5-KDG) eluted out prior to weakly retained compounds (glucose and DGA). Under the optimized conditions, the method showed good linearity in the range of 0.01-20 mg L−1 with determination coefficients (R2)≥99.84%. Low detection limits (LODs) in the range of 0.87-2.59 μg L−1 and good repeatability (RSD<3%, n=6) were obtained. The proposed method has been successfully applied to the analysis of the four compounds in the fermentation broth, in which Gluconobacter oxydans was used to produce gluconic acids from glucose.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 113, 15 September 2013, Pages 113-117
نویسندگان
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