کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7682238 1495819 2013 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Long-tail probe-mediated cycled strand displacement amplification: Label-free, isothermal and sensitive detection of nucleic acids
ترجمه فارسی عنوان
تقویت جابجایی رشته دوچرخه سواری با طول دندانه: شناسایی ایزوترمال، حساس و حساس از اسیدهای نوکلئیک
کلمات کلیدی
پروب طولانی مدت، ایزوترمال تقویت جابجایی رشته ای رشته ای، بدون برچسب
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
We design a long-tail shaped DNA probe for the label-free, isothermal and sensitive detection of nucleic acids based on cycled strand displacement amplification (Ltail-CSDA). The long-tail probe, a stem-loop structure with a long poly(T) tail at 5′-termini, integrates target-binding and amplification and signaling within one multifunctional design. The specific binding between the long-tail probe and the target triggers a polymerization reaction, during which a long dsDNA product is synthesized and the hybridized target is displaced by the strand displacement activity of polymerase. The displaced target forms another specific probe-target binding and prompts cycled polymerization reactions. The proposed Ltail-CSDA has the distinct advantages of its isothermal nature, free-label, simplicity and attomolar sensitivity compared with other existing technologies. More significantly, the dynamic range of the method is extremely large, covering nine orders of magnitude. Using total RNA samples extracted from hepatitis C virus (HCV) as targets, we further demonstrate the detection capability of the method for complex nucleic acid samples, indicating its potential applicability for clinic molecular diagnostic assays.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 116, 15 November 2013, Pages 330-334
نویسندگان
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