Mechanism exploration of adsorption-immobilized enzymatic reactor using polymer-coated silica microbeads

► The efficiency of proteolysis using different charged microbeads was discussed. ► Types of polymer-coated silica beads with opposite charges have been synthesized. ► High digestion efficiency is achieved when both enzyme and protein are adsorbed. ► Electrostatic interaction was considered as the predominant adsorption factor. ► A two-step mechanism for physical immobilized enzymatic reactor is proposed.