Quantum dot-based FRET for sensitive determination of hydrogen peroxide and glucose using tyramide reaction

► A strategy is developed for detection of H2O2 and glucose using QD-based FRET. ► The principle of FRET is based on highly sensitive tyramide reaction. ► Emissions of energy donors and acceptors both appeared on the spectra. ► This method is successfully used for determination of glucose levels in human sera. ► This method is 1 order of magnitude more sensitive than in the commercially method.