Electrochemical determination of cocaine using screen-printed cytochrome P450 2B4 based biosensors

► CYP450 2B4 based biosensors have been developed for cocaine determination. ► The enzyme has been directly screen-printed onto the surface of SPCEs. ► The developed procedure has shown a reproducibility of 5.91% (n=5). ► A capability of detection of 0.2 mM for α=β=0.05 has been yielded. ► The biosensors have been successfully applied to the analysis of cocaine samples.