Extraction and analysis of fungal spore biomarkers in atmospheric bioaerosol by HPLC-MS-MS and GC-MS

► Extraction, purification from particulate matter of three different chemical fungal biomarkers different. ► method provides clean extracts with few matrix effects. ► simultaneous analysis by liquid chromatography and gas chromatography of the three analytes (different in chemical-physical properties). ► Comparison of results between HPLC-MS-MS and GC-MS.