Integrated identification/confirmatory and targeted analysis of epoxyeicosatrienosic acids in human serum by LC-TOF MS and automated on-line SPE-LC-QqQ MS/MS

► We propose a method for qualitative/quantitative targeted analysis of EET in serum.► Identification of EET regioisomers was supported on MS/MS using the hybrid QqTOF. ► The overall automated analytical platform was based on SPE-LC-QqQ MS/MS in SRM.