Development and optimization of a method for the determination of Cylindrospermopsin from strains of Aphanizomenon cultures: Intra-laboratory assessment of its accuracy by using validation standards

► New validated method for CYN determination from lyophilized cyanobacterial cells. ► The method showed to be sensitive, reproducible, accurate, and robust. ► It proves its utility for determining monitoring CYN in lyophilized natural blooms samples by LC-MS/MS.