Influence of buffer composition on the distribution of inkjet printed protein molecules and the resulting spot morphology

► Influence of printing buffers on distribution of inkjet printed biomolecules. ► Distribution of BSA-biotin and IgG-biotin on polystyrene substrate. ► Distribution pattern of biomolecules in buffers of various pH and salt compositions. ► Influence of printing buffers on the real antibody assay performance. ► For printing biomolecules on hydrophobic substrate, carbonate buffer (pH 9.6) was the most suitable.