CdS/TiO2-fluorescein isothiocyanate nanoparticles as fluorescence resonance energy transfer probe for the determination of trace alkaline phosphatase based on affinity adsorption assay

►CdS/TiO2-FITC could emit the fluorescence of both FITC and CdS/TiO2, respectively. ► Fluorescence resonance energy transfer occurred between the CdS/TiO2 and the FITC. ► A CdS/TiO2-FITC-wheat germ agglutinin fluorescence probe was developed. ► The fluorescence probe for the determination of trace AP has been established. ► The reaction mechanism for the determination of AP was also discussed.