Highly sensitive glucose biosensor based on the effective immobilization of glucose oxidase/carbon-nanotube and gold nanoparticle in nafion film and peroxyoxalate chemiluminescence reaction of a new fluorophore

► A novel chemiluminescence (CL) glucose biosensor was constructed. ► Glucose by detected hydrogen peroxide generation in presence of glucose oxidase. ► Gold nanoparticles (GNPs) in nafion film on graphite support enhance detection. ► The linear response range of glucose was found to be 2.25 × 10−6 to 1.75 × 10−4 mol L−1. ► The detection limit was 1.00 × 10−6 mol L−1.