A new derivatization approach for the rapid and sensitive analysis of brassinosteroids by using ultra high performance liquid chromatography-electrospray ionization triple quadrupole mass spectrometry

► A rapid and simple derivatization reaction of brassinosteroids (BRs) using BPBA. ► Sensitive analytical method of BRs with LOD lower to attomolar level. ► Endogenous 24-epiBR and 28-epihomoBR were detected from Arabidopsis thaliana.