The dual role of deposited microbead plug (DMBP): A blood filter and a conjugate reagent carrier toward point-of-care microfluidic immunoassay

► A DMBP with the dual roles: a blood filter and a conjugate reagent carrier. ► DMBP is fabricated by the use of natural deposition of beads. ► Conjugate reagents are incorporated during the assembly of the DMBP. ► Dry conjugate reagents enable to be released from the DMBP in a controlled manner. ► Their activities are retained after 60 days of storage at the room temperature.