Gold nanoparticle-biotinylated liposome hybrids as analytical reagents for biotin determination using a competitive assay and resonance light scattering detection

► Gold nanoparticle-biotinylated liposome hybrids are described as analytical reagents. ► Their usefulness is shown in a competitive assay for biotin determination. ► Resonance light scattering is used as detection system. ► The measurement step is automated by a flow system. ► The method has been satisfactorily applied to the analysis of food samples.