Development of acid phosphatase based amperometric biosensors for the inhibitive determination of As(V)

► Direct amperometric determination of As(V) on AcP based biosensors using SPEs. ► Use of 2-phospho-l-ascorbic acid as a novel substrate for As(V) amperometric analysis. ► Optimization of experimental variables by using experimental design methodology. ► Analysis of the possible effect of the presence of foreign ions in the solution. ► Application of the method to the determination of the As(V) in a ground water sample.