کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7687002 | 1495848 | 2011 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Label-free immunoassay for chloramphenicol based on hollow gold nanospheres/chitosan composite
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Label-free immunoassay for chloramphenicol based on hollow gold nanospheres/chitosan composite Label-free immunoassay for chloramphenicol based on hollow gold nanospheres/chitosan composite](/preview/png/7687002.png)
چکیده انگلیسی
A novel label-free electrochemical immunosensor for rapid determination of chloramphenicol (CAP) was fabricated by entrapping monoclonal antibody to chloramphenicol (anti-CAP) in hollow gold nanospheres (HGNs)/chitosan composite modified on a glassy carbon electrode. The hollow gold nanospheres (HGNs) were prepared by using Co nanoparticles as sacrificial templates and characterized by transmission electron microscopy (TEM). The changes of the electrode behavior after each fabrication step were investigated by electrochemical impedance spectroscopy (EIS) technique. Under optimal conditions, the proposed immunosensor has a sensitive response to CAP in a linear range of 0.1-1000 ng mLâ1 with the detection limit of 0.06 ng mLâ1. Accurate detection of CAP in real meat samples was demonstrated by comparison with conventional HPLC method. The proposed method was proven to be a feasible quantitative method for CAP analysis with the properties of simple preparation, stability, high sensitivity and selectivity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 87, 15 December 2011, Pages 100-105
Journal: Talanta - Volume 87, 15 December 2011, Pages 100-105
نویسندگان
Ningdan Zhang, Fei Xiao, Jing Bai, Yanjun Lai, Jie Hou, Yuezhong Xian, Litong Jin,