Characterization of Pt-protein complexes by nHPLC-ESI-LTQ MS/MS using a gel-based bottom-up approach

► Gel-based bottom-up MS approach for characterizing Pt-protein complexes. ► nrSDS-PAGE separation and conventional in gel digestion preserve Pt-protein bonds. ► Sulfur-containing reagents have a negative impact on Pt-protein bonds preservation. ► Cisplatin binding sites (Met, His, Cys) identified in HSA, TF, CA, MYO and CYT C.